The browser you are using is not supported by this website. All versions of Internet Explorer are no longer supported, either by us or Microsoft (read more here: https://www.microsoft.com/en-us/microsoft-365/windows/end-of-ie-support).

Please use a modern browser to fully experience our website, such as the newest versions of Edge, Chrome, Firefox or Safari etc.

Jens Rydström

Jens Rydström

Professor emeritus

Jens Rydström

Binding of dietary Cobalt to sarcoplasmic reticulum proteins

Author

  • B. Persson
  • E. Carlenor
  • N. Clyne
  • E. Hultman
  • L. E. Lins
  • S. K. Pehrsson
  • J. Rydström

Summary, in English

It has previously been shown that cobalt accumulates in the myocardium of rats, mainly the sarcoplasmic reticulum (SR) and the mitochondrial inner membrane. In order to investigate the mode of accumulation of cobalt in the SR, rats were given a dietary cobalt supplementation of 40 mg of CoSO4 × 7 H2O kg-1 body wt, after which the rats were sacrificed and the sarcoplasmic reticulum was isolated. The SR proteins were subjected to analysis by polyacrylamide gel electrophoresis followed by protein staining and determination of the content of cobalt in each protein band. The major cobalt-binding protein was found to have a molecular weight of about 100 000; a 200 000 molecular weight protein was also found to bind cobalt, although less extensively. These results suggest that cobalt is bound to the monomeric and dimeric forms of Ca2+-ATPase in the SR of the myocardium.

Publishing year

1992

Language

English

Pages

137-140

Publication/Series

Scandinavian Journal of Clinical & Laboratory Investigation

Volume

52

Issue

2

Document type

Journal article

Publisher

Informa Healthcare

Keywords

  • Congestive heart failure
  • Metal ion binding
  • Rat myocardium

Status

Published

ISBN/ISSN/Other

  • ISSN: 0036-5513